Uncovering LINCam
A comprehensive comparison study of wide-field and confocal approaches for single molecule localization microscopy has been published recently. The authors exploit lifetime as a marker to differentiate several chromophores. Surprisingly, LINCam even with a lower quantum efficiency outperforms the confocal method in number of acquired photons per molecule in addition to outstanding signal-to-noise ratio and excellent lifetime accuracy.
- Single-Molecule Fluorescence Lifetime Imaging Using Wide-Field and Confocal-Laser Scanning Microscopy: A Comparative Analysis
- N. Oleksiievets, C. Mathew, J.C. Thiele, J.I. Gallea, O. Nevskyi, I. Gregor, A. Weber, R. Tsukanov and J. Enderlein
- Nano Letters: 22(15), 6454-6461, 2022