Visit us at booth B2.150 at the Laser World of Photonics from 27.06. – 30.06.2023 in München.
Visit us at the DPG Spring Meeting from 05.03. – 10.03.2022 in Hannover.
Single-Molecule Fluorescence Lifetime Imaging Using Wide-Field and Confocal-Laser Scanning Microscopy: A Comparative Analysis
A comprehensive comparison study of wide-field and confocal approaches for single molecule localization microscopy has been published recently. The authors exploit lifetime as a marker to differentiate several chromophores. Surprisingly, LINCam even with a lower quantum efficiency outperforms the confocal method in number of acquired photons per molecule in addition to outstanding signal-to-noise ratio and excellent lifetime accuracy.
Visit us at the booth of Omicron at the Laser World of Photonics from 26.04. – 29.04.2022 in München.
Large scale FLIM by optical sectioning of rat embryo. When the size matters.
Stored ions are typical examples for single photon sources that show an antibunched emission statistics. However if two stored ions are brought in an entangled state by simply measuring, interesting phenomena occur.
Confocal superresolution FLIM microscopy with a spinning disk unit
By using the metabolite NADH as an intrinsic marker for glycolysis, the dynamics of individual cells can be monitored and their interactions studied.
Metal induced energy transfer (MIET) is a nanoscopy technique to measure distances from fluorescent molecules to a metal film. Here we present MIET acquisition using wide-field and TIRF fluorescence lifetime imaging microscopy (FLIM) equipped with LINCam.